Preliminary findings suggest HB grafts are effective for bone regeneration with significant potential for clinical translation. Studies of hemoglobin subcutaneous implants using an in vivo mouse model, have demonstrated that HB does not illicit an immune response that could be detrimental to implant success, avoiding comprising materials rapidly vascularizing and integrating with host tissue. Adult male Sprague Dawley rats had critical-sized calvarial defects which were left either unfilled as control defects or filled with a single full-thickness piece of autogenous bone, HB scaffold, or empty scaffold. The defects were left to heal for 8 weeks postoperatively before terminal perfusion with Microfil. CT scans of the calvarial specimens were performed. Histomorphometric assessment of hematoxylin-eosin stained specimens was used to analyze the proportion of new bone and blood vessels in the calvarial defects. The ongoing study using in vivo rat calvarial model, showed higher new bone volume as a percentage of total volume in calvarial defects treated with the HB scaffolds compared to empty defects or empty scaffolds.
- Understand the medical need for osteogenic biomaterials that are not only efficacious but also easy to surgically implement, cost-effective, and capable of being manufactured on scales to address this need.
- Appreciate how Hyperelastic Bone is created from well-established, safe, clinically used medical materials with unique mechanical, physical, and biological properties that can be synthesized and manufactured for clinical needs.